Journal: Cell Communication and Signaling : CCS

Article Title: Neuropilin-1 drives tumor-specific uptake of chlorotoxin

doi: 10.1186/s12964-019-0368-9

Figure Lengend Snippet: ER-472 antitumor activity and levels of active metabolite in 3 xenograft models; MIA PaCa-2, BxPC-3 and PC-3. Subcutaneous tumors were established in nude mice; treatment groups were n = 6 for MIA PaCa-2 and BxPC-3 and n = 5 for PC-3 efficacy studies and n = 3 for all metabolite studies. a , ER-472 was administered i.v. Q4Dx3 (dosing days denoted with long ticks on x-axis). Tumor volume data represents the mean ± SEM. b , mean active metabolite (S-methyl cryptophycin) tumor concentration versus time following a single dose of ER-472 at 2.5 mg/kg (MTD). Data represents the mean ± StdDev. c , antitumor activity of ER-472 versus ER-271 at molar equivalent (ME) dose in PC-3 model. * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, one-way ANOVA followed by Tukey’s multiple comparison test; n.s. = not significant

Article Snippet: BxPC-3 cells (BW125058) were from Perkin Elmer (Waltham, MA), MIA PaCa-2 (ATCC Cat# CRM-CRL-1420, RRID:CVCL_0428) and PC-3 (ATCC Cat# CRL-7934, RRID:CVCL_0035) were from ATCC.

Techniques: Activity Assay, Concentration Assay

Journal: Cell Communication and Signaling : CCS

Article Title: Neuropilin-1 drives tumor-specific uptake of chlorotoxin

doi: 10.1186/s12964-019-0368-9

Figure Lengend Snippet: Correlation of ER-472 antitumor activity to NRP1 expression in tumor lysates in multiple xenograft models. Antitumor activity was assessed for ER-472 administered i.v. on a Q4Dx3 schedule, at ¼ to full MTD doses in multiple xenograft models. NRP1 expression in tumors was assessed by mRNA analysis and/or protein detection by western blot or IHC. Reasonable correlation was determined for 11 of the 13 models

Article Snippet: BxPC-3 cells (BW125058) were from Perkin Elmer (Waltham, MA), MIA PaCa-2 (ATCC Cat# CRM-CRL-1420, RRID:CVCL_0428) and PC-3 (ATCC Cat# CRL-7934, RRID:CVCL_0035) were from ATCC.

Techniques: Activity Assay, Expressing, Western Blot

Journal: International Journal of Bioprinting

Article Title: Bioprinting of hydrogel beads to engineer pancreatic tumor-stroma microtissues for drug screening

doi: 10.18063/ijb.676

Figure Lengend Snippet: Schematic presentation of this study. Three-dimensional pancreatic ductal adenocarcinoma (PDAC) models were fabricated by bioprinting of pancreatic cancer BxPC-3 cells and normal human dermal fibroblast cells laden-GelMA hydrogel beads using the dot extrusion printing technology. Drug treatment on the uniform-sized PDAC models was conducted after 1-week culture.

Article Snippet: Human pancreatic cancer cell line (BxPC-3) and normal human dermal fibroblast cells (NHDFs) in this work were kindly provided by Hangzhou Regenovo Biotechnology Co., Ltd. For cells cultivation, BxPC-3 and NHDFs were cultured in RPMI-1640 and Dulbecco’s modified Eagle’s medium (DMEM; Gibco, USA), respectively.

Techniques:

Journal: International Journal of Bioprinting

Article Title: Bioprinting of hydrogel beads to engineer pancreatic tumor-stroma microtissues for drug screening

doi: 10.18063/ijb.676

Figure Lengend Snippet: Cell viability analysis of the engineered three-dimensional pancreatic ductal adenocarcinoma (PDAC) models. (A) Representative fluorescent micrographs of three different PDAC models including mono-tumor, stroma-poor and stroma-rich microtissues at days 1, 4, and 7. Scale bar = 200 μm. (B) Cell viability. Experimental values are expressed in mean ± standard error, n = 3.

Article Snippet: Human pancreatic cancer cell line (BxPC-3) and normal human dermal fibroblast cells (NHDFs) in this work were kindly provided by Hangzhou Regenovo Biotechnology Co., Ltd. For cells cultivation, BxPC-3 and NHDFs were cultured in RPMI-1640 and Dulbecco’s modified Eagle’s medium (DMEM; Gibco, USA), respectively.

Techniques:

Journal: International Journal of Bioprinting

Article Title: Bioprinting of hydrogel beads to engineer pancreatic tumor-stroma microtissues for drug screening

doi: 10.18063/ijb.676

Figure Lengend Snippet: Morphology and organization of cells embedded in GelMA hydrogel beads of different pancreatic ductal adenocarcinoma (PDAC) models during 1-week culture. (A) F-actin staining of cell morphology and structure at day 1, 4 and 7. Green channel: F-actin. Blue channel: DAPI. Scale bar = 100 μm. (B) Assessment of the proliferation of different PDAC microtissues. (C) Assessment of the density of tumor microstructure. Experimental values are expressed in mean ± standard error, n = 3. Two-way ANOVA, * P < 0.05, *** P < 0.001, **** P < 0.0001.

Article Snippet: Human pancreatic cancer cell line (BxPC-3) and normal human dermal fibroblast cells (NHDFs) in this work were kindly provided by Hangzhou Regenovo Biotechnology Co., Ltd. For cells cultivation, BxPC-3 and NHDFs were cultured in RPMI-1640 and Dulbecco’s modified Eagle’s medium (DMEM; Gibco, USA), respectively.

Techniques: Staining

Journal: International Journal of Bioprinting

Article Title: Bioprinting of hydrogel beads to engineer pancreatic tumor-stroma microtissues for drug screening

doi: 10.18063/ijb.676

Figure Lengend Snippet: Tunable tumor-stroma microenvironment profiling. (A) Immunostaining of BxPC-3 cells and normal human dermal fibroblast cells with CK19 and α-SMA in different pancreatic ductal adenocarcinoma (PDAC) models at day 1, and 7. Green channel: α-SMA. Red channel: CK19. Blue channel: DAPI. Scale bar = 200 μm. (B) Quantitation of α-SMA expression in the co-culture models. (C) Quantitation of CK19 expression in different PDAC models. Experimental values are expressed in mean ± standard error, n = 3. Two-way ANOVA, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

Article Snippet: Human pancreatic cancer cell line (BxPC-3) and normal human dermal fibroblast cells (NHDFs) in this work were kindly provided by Hangzhou Regenovo Biotechnology Co., Ltd. For cells cultivation, BxPC-3 and NHDFs were cultured in RPMI-1640 and Dulbecco’s modified Eagle’s medium (DMEM; Gibco, USA), respectively.

Techniques: Immunostaining, Quantitation Assay, Expressing, Co-Culture Assay

Journal: International Journal of Bioprinting

Article Title: Bioprinting of hydrogel beads to engineer pancreatic tumor-stroma microtissues for drug screening

doi: 10.18063/ijb.676

Figure Lengend Snippet: Drugs screening performed in different three-dimensional pancreatic ductal adenocarcinoma (PDAC) models after 7 days of culture. (A) Representative fluorescent micrographs of PDAC models after incubation with gemcitabine ranging from 50 to 100 μM/mL for 72 h. Non-drug-treated microtissues were observed as controls. Green channel: Calcein-AM. Red channel: PI. Scale bar = 200 μm. (B) Statistical plot of cell viabilities after treating PDAC models with different concentrations of gemcitabine for 72 h. (C) Heat map representing the drug treatment results. Experimental values are expressed in mean ± standard error, n = 3. Two-way ANOVA, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

Article Snippet: Human pancreatic cancer cell line (BxPC-3) and normal human dermal fibroblast cells (NHDFs) in this work were kindly provided by Hangzhou Regenovo Biotechnology Co., Ltd. For cells cultivation, BxPC-3 and NHDFs were cultured in RPMI-1640 and Dulbecco’s modified Eagle’s medium (DMEM; Gibco, USA), respectively.

Techniques: Incubation